Part:BBa_K371053:Design

theRFC 10 and RFC53 interface converter

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 14
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI site found at 3
  

Design Notes

In the middle of this part is a NheI site, which is designed as a mean to examine the system.

All from 5' to 3'

MPF:GAGCTCTTCA

MSF:TGAAGAGATC

NheI:GCTAGC

Source

Since the sequence is not very long, you can easily get it by direct synthesis.